Applied Sciences grants Manar M. Rashid Master gegree about thesis entitled “Preparation and Characterization of Metallic Nanoparticles Loaded with Lipopolysaccharide Isolated from Iraqi Hospitals for Theranostic Study”.
The discussion committee consisted of Dr. Sanad Mohammed Baqer / Biochemistry - University of Baghdad, Dr. Majid Sakhi Jaber / Immunology - University of Technology and Dr. Intisar Hussein Ali / Enzimat - University of Technology as members and Dr. Ali Abdul Rahman Taha / fungi and poisons - University of Technology supervisor Nada Hisham Abdel-Latif / Biotechnology as Co-supervisor.
The study concluded “Lipopolysaccharide (LPS) is an essential component of the outer membrane of Gram-negative bacteria, playing an important role in the protection of harsh environments, in drug resistance and in pathogenesis. In the present study,25 urine samples were collected from patients with urinary tract infection from the Central Teaching Hospital of Peadiatric in Baghdad/Iraq. Macroscopic, microscopic and Api20E system were used to identify Escherichia coli from15 suspectedisolates. One E. coli isolate was chosen to produce and extract LPS. The LPS was extracted and purified by hot phenol method followed by Sephacryl S-200 gel filtration chromatography to obtain 2.5 mg of LPS. Moreover, molecular weight of LPS was 105KD estimated after gel filtration.
On the other hand, CuS NPs had been synthesized by bovine serum albumin (BSA) entrapment characterized by UV/Visible spectrophotometer,SEM,XRD,FTIR and Zeta potential. The results revealed an absorbance peaks at 197 nm and 616 nm due to the presence of BSA and CuS NPs respectively. The particle size of CuS-BSA NPs ranging between 30–60 nm was observed and analyzed by SEM.The EDX analysis was used to determine the chemical composition of CuS-BSA NPs and the results showed the presence alarge percentageof carbon in addition to the oxygen, nitrogen, copper and sulfur.Furthermore, the FTIR spectrum result of CuS-BSA NPs was shown the N–H bond of amino groups and a hydroxyl group (-OH) at 3435.34 cm-1, while the (S-H) band was seen at 2729.37 cm-1. The zeta potential value of CuS-BSA NPs was (-14.49 mV).
The CuS-BSA nanoparticles showed an antibacterial activity against S.aureus,Proteus, E. coli and Enterobacterbacteriaand the mean of inhibition zone diameter was (10, 8, 7, 6) respectively.In order to confer the specificity and enhancethe antitumor activity, the TAT peptide and LPS had been combined with CuS NPs. The cytotoxic effects of CuS-BSA NPs, LPS, CuS-BSA+LPS and CuS + BSA + LPS + TAT against RD and L20B cell lines were determined by MTT assay.The results showed a significant cytotoxic effect on RD cell line (P <0.001), while there were high effects (56 %) when 1 µg/ml CuS + BSA + LPS + TAT was examined.On the other hand, the low cytotoxicity percentage of LPS, CuS-BSA NPs and CuS-BSA+LPS against L20B cells were observed, while after binding with TAT peptide, the cytotoxicity percentage increased to 61.52% with highly significant effect (P˃0.001)”.