Toxic effects of fabricated gold nanoparticles in albino mice
Azhar J. Al- Mosowy , Ali A. Taha , Lames TH. AL-Hadedee
Abstract

Polyethylene glycol (PEG) is commonly used to functionalize the surface of gold nanoparticles (GNPs) in order to improve their in vivo stability. This study investigates the in vivo toxicity of GNPs coated with PEG in mice. Gold nanoparticles were fabricated by chemical method technique, it is based on reduction of HAuCl4. GNPs were coated with Polyethylene glycol (PEG). The product was characterized by scanning electron microscope (SEM) and UV–vis absorbance spectra. Scanning electron microscopy showed formation of gold nanoparticles in spherical shapes. The average size of the gold nanoparticles was 75.2 nm and 76.6 nm for GNPs coated with PEG. UV–vis absorbance spectra results show that the optical absorption in λ 519 nm and 525.5 nm for fresh GNPs and GNPs coated with PEG respectively. Zeta potential measurements were -26.55 mV. -8.2 mV for GNPs with PEG respectively, which are sufficient to prevent nanoparticles aggregation. 45 male albino mice were randomly divided into 3 groups, injected intraperitoneally with GNPs at doses of 0.1 ml containing 0.01 IU/10g mouse weight (group A), 0.1 ml of GNPs coated with PGD containing 0.01 IU/10g of mouse weight (group B) and 0.1 ml of distilled water/10g of mouse weight (control group) every 48 hours for 2 months. Our results show that GNPs cause change in texture of spleen, brain, liver, and kidney in vivo. However, toxicity indicates that PEG coated GNPs was less than GNPs without PEG.
Key words; Gold nanoparticle, size, mice, toxicity, polyethylene glycol
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