The Effect of Different Culture Media on In Vitro Fertilization of Mice
Mohammed A. Ibrahim , Hazim I. Al-Ahmad , Wasnaa H. Mohammed
Abstract

The effect of different types of media on in vitro fertilization (IVF) of mice oocyte was investigated. IVF was carried out in RPMI-1640 medium at recommended temperature (37 C) and pH (7.4). The obtained results showed that 63.73% of used oocytes were fertilized, and the percentage of abnormal embryos was 27.58%. While, using other culture media at same temperature and pH, gave low percentage of fertilization and higher yield of abnormal embryos. Thus, using Medicult IVF, Earl s and TCM-199 were gave fertilization percentages equivalent to 38.75, 42.42 and 61.45% respectively; whereas, the percentages of abnormal embryos were 32, 35.7 and 33.89 % respectively. The reported results indicate that composition of various types of culture media have significant effect on the rate of fertilization and abnormal embryos.
Keywords: culture medium, in vitro fertilization, IVF.
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