Synthesis and Antibacterial Activity of CuO Nanoparticles Suspension Induced by Laser Ablation in Liquid
Khawla S. Khashan , Ghassan M. Sulaiman , Farah A. Abdulameer

In the present study, copper oxide (CuO) colloidal nanoparticles (NPs) were synthesized using laser ablation of copper pellet immersed in deionized water. Pulsed Nd: YAG laser was used to irradiate the targets at different laser energies and various ablation times. NP suspensions were characterized by UV–Vis spectroscopy, XRD, and TEM. The absorption spectrum exhibited a peak at ∼275nm and another peak with low intensity at ∼645nm. The XRD pattern of the NPs proved the presence of (–111) and (112) planes assigned to the CuO phase. The TEM images showed nearly spherical shape nanoparticles CuO NPs with size of 3–40nm. The antibacterial activity of CuO NPs was first carried out against four types of bacteria: Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris and Staphylococcus aureus, by liquid medium method. CuO NPs showed the highest antibacterial activity against E. coli at the highest concentration (1000 µg mL−1). CuO NPs and amoxicillin had a synergistic effect on inhibiting E. coli and S. aureus growth; this effect was also tested using the well diffusion method. In this method, CuO NPs at a concentration of 1000 µg mL−1 along with amoxicillin showed the inhibition zone against E. coli (26.0 ± 1.00 mm), as well as complete inhibition of bacteria against S. aureus.
Keywords CuO · Nanoparticles · PLAL · X-ray diffraction · TEM · Antibacterial activity
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